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The denatured proteins were mixed with 300 µL 0.22-µm-pore filtered UA buffer (8 M urea, 0.1 M TEAB, pH 8.5) and then transferred to a 30-kDa centrifugal filter (Millipore, Billerica, MA, USA)
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Changes in brain metabolism might contribute to these phenotypes, as many of the deleted genes are involved in metabolic processes, but this is unknown
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Check whether a remaining issue needs to stay in the same case. Close the request when the documented issue has been suitably addressed.
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