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Internalization, ADC cytotoxicity, and TCE cytotoxicity assays For internalization assays, cells (SW48, BXPC3, PC3, bronchial/tracheal epithelial cells and cervical epithelial cells) were grown in manufacturer’s recommended media and were collected and plated at 20,000 cells per well in a tissue culture treated 96-well plate
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For the results of RBD proteins binding to ACE2 expressed in HeLa cells, the significance of differences between the groups was determined using one-way ANOVA followed by Dunnett’s multiple comparisons tests
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