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547 Various laboratory techniques, such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), allele-specific oligonucleotide polymerase chain reaction (ASO-PCR), single-strand conformation polymorphism (SSCP), long-range PCR, and Southern blot, have traditionally been used to screen for point mutations and deletions in mtDNA

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& Hesketh, J