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The constructs were amplified from the previously described hTrxR1 U498C construct, using OhTrxRf (5′-TATTACATATGAACGGCCCTGAAGATCTTCCCAAGTCC-3′) as forward primer, and OhTrxRrSC (5′-TATTAAAGCTTTTAACCACAGGAGCCAGCCTGGAGGAT-3′), OhTrxRrCS (5′-TATTAAAGCTTTTAACCAGAGCAGCCAGCCTGGAGGAT-3′), or OhTrxRrCtrunc (5′-TATTAAAGCTTTTAACCTTAGCAGCCAGCCTGGAGGAT-3′) as reverse primers for PCR, respectively 33
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