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Unlike plain sterile water, the preservative helps inhibit bacterial growth after the vial has been punctured, allowing multiple withdrawals when proper sterile laboratory techniques are followed
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The protein samples (12 µL) and Spectra Multicolor Broad Range Protein Ladder (7.5 µL) with 10–260 kDa range (Thermo Fisher Scientific) were loaded onto the gel, and electrophoresis was run at 200 V for 22 min in 1× Bolt MES SDS Running Buffer (Invitrogen)
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