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This assay utilized recombinant human CSNK1A1 and peptides corresponding to the hinge region of NHR-49 as the substrate to test for direct phosphorylation
Peptide Sciences
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13 C NMR (151 MHz, DMSO-d 6 ) δ 178.3 (PtO C (=O)CH 3 ), 173.2 (B-10), 163.9, 163.8 (d, B-9), 163.4, 163.4 (PtO C (=O) 2 ), 61.1, 60.8 (D-2), 53.4, 53.2 (d, B-4), 52.9, 52.9 (d, B-7), 50.7, 50.6 (d, B-5), 31.0, 30.9 (D-3), 24.7, 24.6 (d, B-6), 24.1, 24.0 (d, B-3), 23.5, 23.5 (D-4), 22.9 (PtOC(=O) C H 3 ), 21.1 (B-2), 13.6 (B-1)
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