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Cells were seeded at a density of 1.5×105 cells/well into 6-well plates, cultured overnight and treated with RSL3 or Erastin (A549 cells were treated with 2 μM RSL3 or 20 μM Erastin and H460 cells were treated with 8 μM RSL3 or 20 μM Erastin) at 37°C for 24 h
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