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In the case of non-essential genes, most of them causing minimal growth defects when disrupted, the discernible phenotype has to be identified in mutant strains or in assays under restrictive media conditions, such as the following: growth in non-fermentable carbon source for genes affecting mitochondrial functions [21], absence of metabolite for gene involved in metabolic pathway [21,22], adding chemicals to sensitize the yeast strain for genes involved in DNA repair [23,24], or converting the non-essential yeast gene to an essential gene by disrupting a synthetic lethal partner [25]

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This will help ensure the ingredients stay evenly mixed